This Healthy Aging Seminar is co-sponsored by the UBC Department of Biochemistry and Molecular Biology.
Title: Mapping Genetic Interaction Networks in Yeast and Human Cells
Summary: We’ve generated a comprehensive genetic network in yeast cells, testing all possible 18 million gene pairs for genetic interactions. Negative interactions connected functionally related genes and coherent sets of genetic interactions connect protein complex and pathway modules to map a functional wiring diagram of the cell. The global genetic network can be exploited to interpret chemical-genetic profiles associated with bioactive compounds, linking them to their cellular target pathways. We’ve examined trigenic interaction networks and how different environments modulate the global yeast genetic interaction network. We’ve also mapped genetic suppression networks on a large-scale in yeast. To test for conservation of the general principles of genetic networks, we are utilizing CRISPR-Cas9 technology to conduct genome-wide screens and map genetic interactions in haploid human cells.
Speaker Biography: Dr. Charlie Boone received his PhD in Biology from McGill University and completed postdoctoral studies at the Institute of Molecular Biology, University of Oregon. He is recognized for his invention of innovative methods for large-scale mapping of genetic and chemical-genetics networks in the yeast model system. He has also pioneered methods for using genetic interactions to discover cellular targets of bioactive molecules. The RIKEN Institute (Tokyo) has recognized Dr. Boone’s contributions in the field of chemical genomics through his appointment as a team leader in chemical genetics in the Centre For Sustainable Resource Science. Dr. Boone received the Genetics Society of America (GSA) Edward Novitski Prize, creativity in genetics.
Please register here for this seminar. This seminar can ONLY be viewed in-person in Room 102 of Michael Smith Laboratories at UBC (i.e., virtual attendance is not provided).
